UCH-L1 Deubiquitinase Inhibitor Screening Kit

• Cat.No.: Kit-2110
• Product Overview: The UCHL1 Inhibitor Screening Kit utilizes the ability of active UCH-L1 deubiquitinase to cleave a synthetic protein substrate to release the free fluorophore, which can be easily quantified (Ex/Em = 350/440 nm) using a fluorescence microplate reader. Small molecule inhibitors can either reduce or abolish this activity. This inhibitor screening kit thus allows rapid and reliable determination of the inhibitory effects of various compounds on UCH-L1 deubiquitinase and should be used to screen for novel inhibitors.

Specification

• Size: 100 assays
• Applications: Screening potential inhibitors/ligands of UCH-L1Characterizing/studying UCH-L1 inhibitors
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.UCH-L1 Assay Buffer: Ready to use. Warm to room temperature before use. Store at -20°C.UCH-L1 Substrate: Warm UCH-L1 Substrate to room temperature before use. Light sensitive. Store at -20°C. Use within two months.UCH-L1 Enzyme: Reconstitute with 220 µl of UCH-L1 Assay Buffer to prepare the stock solution. Aliquot & store at -80°C. Avoid repeated freeze/thaw. Use within two months.UCH-L1 Inhibitor (LDN-57444): Inhibitor is supplied to allow user to validate experimental set up and assure that screening will identify inhibitors. There is enough inhibitor for 10 assays.