"Nuclei" Related Products

Yeast Nuclei Isolation Kit

Cat.No.: Kit-0637
Product Overview: Yeast Nuclei Isolation Kit is used for isolating yeast nuclei from yeast cell cultures.
Description: To understand the nature of the replication and expression of the yeast genome, it is essential to have a method for the preparation of nuclei at different stages of growth. Yeast Nuclei Isolation Kit enables fast and easy purification of nuclei from yeast cells, utilizing yeast cell wall lysis and homogenization.
Applications: DNA-Protein interaction, RNA-Protein interaction and Protein-Protein interaction studiesDNase I footprinting analysis, Enzymatic Assays and Pull-down assayWestern blot
Target Species: Yeast
Usage: For research use only (RUO)
Storage: Store kit at -20°C. Warm Buffers A and B to room temperature (RT) before use.
Kit Components: Buffer A 50 mLBuffer B 50 mL1 M DTT 1 mLLysis Enzyme Mix 500 μLBuffer N 90 mLProtease Inhibitor Cocktail (Lyophilized) 1 vial
Compatible Sample Types: Cell culture

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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