"6-PGD" Related Products

        6-Phosphogluconate Dehydrogenase Activity Colorimetric Assay Kit

        Cat.No. : Kit-2208
        Product Overview : 6-Phosphogluconate dehydrogenase (6-PGD, EC is an enzyme in the pentose phosphate pathway that catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose-5-phosphate with simultaneous reduction of NADP+ to NADPH. Ribulose-5-phosphate is used for production of nucleotides and nucleic acids and can also be reversibly converted to glyceraldehyde-3-phosphate and fructose-6-phosphate which can be used in the glycolysis pathway. It also has a vital role in production of NADPH, which is utilized as a reducing agent in numerous biosynthetic pathways and is important in prevention of oxidative damage. In humans, 6-PGD deficiency is an autosomal hereditary disorder that can result in depletion of NADPH in red blood cells. As NADPH is required for regeneration of the cellular antioxidant glutathione in red blood cells, 6-PGD deficiency increases the risk of hemolytic anemia in situations of oxidative stress. On the other hand, inhibition of 6-PGD has been linked to anti-cancer activity by decreasing RNA biosynthesis and increasing the buildup of reactive oxygen species in tumor cells. 6-PGD assay kit provides a quick, sensitive and easy way for measuring 6-PGD activity in various biological samples. In this assay, 6-PGD converts 6-phosphogluconate into an intermediate, generating NADPH, which subsequently reduces a colorless probe into a strongly colored product detectable by absorbance at 460 nm. The assay is high-throughput adaptable and can detect less than 0.05 mU of 6-PGD activity.
        Applications : Measurement of 6-PGD activity in purified/crude enzyme preparations and biological samples.
        Storage : -20°C
        Shipping : Gel Pack
        Size : 100 assays
        Kit Components : 6-PGD Assay Buffer; 6-PGD Substrate; 6-PGD Developer; NADH Standard; 6-PGD Positive Control
        Target Species : Mammalian
        Detection method : Colorimetric (OD 460 nm)
        Features & Benefits : Simple, rapid & convenient assay to measure 6-PGD Activity in biological samples;
        Includes Positive Control

        Online Inquiry

        Note: There will be extra charge for optional service!

        Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

        Optional requirements on this protein    +Expand

        Price Inquiry

        Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.