"7-AAD/CFSE" Related Products

7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit

Cat.No.: Kit-1919
Size: 1 ea
Description: The 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit employs CFSE to label target cells within the mixed cell population and 7-AAD to label dead cells. This kit provides an improvement over the traditional 51chromium (51Cr) release assay to assess cell-mediated cytotoxicity. CFSE labeling is more sensitive, does not employ radioisotopes, and cytolysis can be assessed at the single-cell level. The mixed lymphocyte reaction is a cornerstone method of immunology1, and this kit can help immunologists to detect subtle changes in cytotoxic lymphocyte function. The kit provides sufficient reagents to effectively stain approximately 10^9 cells.
Storage: -20°C
Kit Components: Cell-Based Assay 7-AAD Staining Stock Solution (1,000X): 2 vials/50 µl; 4°C CFSE Stock Solution: 1 vial/100 µl; -20°C Cell-Based Assay Buffer Tablet: 4 Tablets; RT

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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