Recombinant Mouse Wnt5a Protein, Gln38-Lys380

• Cat.No.: WNT5A-19H
• Product Overview: Recombinant human/Mouse Wnt5a protein (Gln38-Lys380) without tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Mouse
• Source: CHO
• Protein Length: 38-380 a.a.
• Description: Enables frizzled binding activity; protein domain specific binding activity; and receptor ligand activity. Involved in several processes, including Wnt signaling pathway; heart development; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including animal organ development; positive regulation of protein phosphorylation; and regulation of animal organ morphogenesis. Located in cell surface and extracellular matrix. Is expressed in several structures, including alimentary system; embryo ectoderm; genitourinary system; limb; and sensory organ. Used to study Robinow syndrome. Human ortholog(s) of this gene implicated in autosomal dominant Robinow syndrome 1. Orthologous to human WNT5A (Wnt family member 5A).
• Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 μg/mL, in the presence of 5 ng/mL rmWnt-3a. Optimal concentrations should be determined by each laboratory for each application.
• Molecular Mass: Predicted Molecular Mass: 38 kDa
  SDS-PAGE: 45 kDa, reducing conditions
• Endotoxin: < 1 EU/μg by LAL
• Purity: > 80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• N-terminal Sequence Analysis: Asn44 & No results obtained: Gln38 predicted
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Wnt5a wingless-type MMTV integration site family, member 5A [ Mus musculus (house mouse) ]
• Official Symbol: Wnt5a
• Synonyms: Wnt5a; wingless-type MMTV integration site family, member 5A; Wnt-5a; 8030457G12Rik; protein Wnt-5a; wingless-related MMTV integration site 5A
• Gene ID: 22418
• mRNA Refseq: NM_009524
• Protein Refseq: NP_033550
• UniProt ID: P22725