| Source : |
HEK293 |
| Species : |
Canine |
| Tag : |
N-hFc |
| Protein length : |
Pro285-Lys410 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by its ability to inhibit NGF-induced prolifeRation of TF?1 Human erythroleukemic cells.The ED50 for this effect is typically 2-8 μg/mL in the presence of 10 ng/mL of recombinant Human NGF. |
| Molecular Mass : |
The recombinant Canine NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 386 amino acids and has a predicted molecular mass of 42.3 kDa. The apparent molecular mass of the protein is approximately 55-61 kDa in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 90 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Official Symbol : |
NTRK1 |
