Acitve Recombinant Human CD27 protein(Met1-Ile192), His-tagged

• Cat.No.: CD27-1345H
• Product Overview: Recombinant Human CD27(P26842) ( Met 1-Ile192) was expressed in Insect Cells with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Ile192
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD27-his at 10 μg/mL (100 μl/well) can bind biotinylated Human CD70-Fc, The EC50 of biotinylated Human CD70-Fc is 57-85 ng/mL.
• Molecular Mass: The secreted recombinant Human CD27 consists of 184 amino acids and predicts a molecular mass of 20.77 KDa. The apparent molecular mass of the protein is approximately 27 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD27 CD27 molecule [ Homo sapiens ]
• Official Symbol: CD27
• Synonyms: CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393
• Gene ID: 939
• mRNA Refseq: NM_001242
• Protein Refseq: NP_001233
• MIM: 186711
• UniProt ID: P26842