Acitve Recombinant Human EPOR & CD131 protein(Met1-Pro250 & Trp17-Trp443), hFc-tagged

• Cat.No.: EPOR & CD131-1383H
• Product Overview: Recombinant Human EPOR (NP_000112.1)(Met 1-Pro 250) linked with the extracellular domain (Trp 17-Trp 443) of Human CD131 (NP_000386.1) by a peptide linker was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Pro250 & Trp17-Trp443
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind Rat EPO-His in a functional ELISA. 2. Measured by its ability to bind Mouse EPO-His in a functional ELISA.
• Molecular Mass: The recombinant heterodimer of Human EPOR & CD131 comprises 906 amino acids and has a calculated molecular mass of 101 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPOR erythropoietin receptor [ Homo sapiens ]
• Official Symbol: EPOR; CD131
• Synonyms: EPOR; erythropoietin receptor; EPO-R; MGC138358
• Gene ID: 2057
• mRNA Refseq: NM_000121
• Protein Refseq: NP_000112
• MIM: 133171
• UniProt ID: P19235