Acitve Recombinant Human FGFR1 protein(Met1-Lys310), hFc-tagged

• Cat.No.: FGFR1-1275H
• Product Overview: Recombinant Human FGFR1 alpha (IIIb) (NP_056934.2) (Met1-Lys310 and Ala359-Glu374 linked by FGFR1 IIIb Ig domain (AAB19502.1) (His1-Pro47)) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-hFc
• Protein length: Met1-Lys310
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit FGF-acidic dependent prolifeRation of Balb/c 3T3 Mouse fibroblasts. The ED50 for this effect is typically 0.1-0.6 ng/mL
• Molecular Mass: The recombinant Human FGFR1 alpha (IIIb) consists of 590 amino acids and predicts a molecular mass of 65.7 kDa. As a result of glycosylation, it migRates as an approximately 113.3 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FGFR1 fibroblast growth factor receptor 1 [ Homo sapiens ]
• Official Symbol: FGFR1
• Synonyms: FGFR1; fibroblast growth factor receptor 1; FLT2, fms related tyrosine kinase 2 , KAL2; BFGFR; CD331; CEK; FLG; H2; H3; H4; H5; N SAM; Pfeiffer syndrome; FGFR1/PLAG1 fusion; proto-oncogene c-Fgr; FMS-like tyrosine kinase 2; hydroxyaryl-protein kinase; fms-related tyrosine kinase 2; heparin-binding growth factor receptor; basic fibroblast growth factor receptor 1; OGD; FLT2; KAL2; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; bFGF-R-1; FLJ99988
• Gene ID: 2260
• mRNA Refseq: NM_001174063
• Protein Refseq: NP_001167534
• MIM: 136350
• UniProt ID: P11362