Acitve Recombinant Human SERPINB3 protein, His-tagged

• Cat.No.: SERPINB3-1335H
• Product Overview: Acitve Recombinant Human SERPINB3 protein(AAB20405.1) (Asn 2-Pro 390) was expressed in Insect Cells, with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 2-390 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT.
• Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenice ptide substrate Z-LR-AMC. The IC50 is < 5 nM.
• Molecular Mass: The recombinant human SERPINB3 consists of 408 amino acids and predicts a molecular mass of 46.7 kDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SERPINB3 serpin peptidase inhibitor, clade B (ovalbumin), member 3 [ Homo sapiens ]
• Official Symbol: SERPINB3
• Synonyms: SERPINB3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; SCC, SCCA1, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; serpin B3; HsT1196; T4 A; protein T4-A; squamous cell carcinoma antigen 1; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; SCC; T4-A; SCCA1; SCCA-1; SCCA-PD
• Gene ID: 6317
• mRNA Refseq: NM_006919
• Protein Refseq: NP_008850
• MIM: 600517
• UniProt ID: P29508