Acitve Recombinant Mouse ICOS protein(Met1-Gln135), His-tagged

• Cat.No.: ICOS-1248M
• Product Overview: Recombinant Mouse ICOS (NP_034643.2) (Met1-Gln135) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gln135
• Form: Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Mouse ICOS Protein (His Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Mouse ICOS Ligand / B7-H2 / ICOSLG Protein (His & Fc Tag), The EC50 is 60-160 ng/mL.
• Molecular Mass: The recombinant Mouse ICOS consists of 121 amino acids and predicts a molecular mass of 13.9 kDa. As a result of glycosylation, it migRates as an approximately 22.7 and 19.7 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Icos inducible T cell co-stimulator [ Mus musculus ]
• Official Symbol: ICOS
• Synonyms: ICOS; inducible T cell co-stimulator; inducible T-cell costimulator; CD28-related protein 1; CD28 and CTLA-4-like protein; inducible T-cell co-stimulator; activation-inducible lymphocyte immunomediatory molecule; H4; CCLP; AILIM; CRP-1; Ly115
• Gene ID: 54167
• mRNA Refseq: NM_017480
• Protein Refseq: NP_059508