|Product Overview:||Aconitase (aconitate hydratase; EC 220.127.116.11) is an iron-sulfur protein containing an [Fe;S;]2; cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.|
|Kit Components:||Assay Buffer; Substrate; Developer; Enzyme Mix (200μl); Cysteine; (NH4)2Fe(SO4)2; Isocitrate Standard (10μmol)|
|Detection method:||Absorbance at 450 nm|
|Features & Benefits:||Simple procedure; takes ~ less than 40 minutes; Fast and convenient|
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