Aconitase Activity Colorimetric Assay Kit

• Cat.No.: Kit-2265
• Product Overview: Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe;S;]2; cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.

Specification

• Tag: Non
• Storage: 4°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: Assay Buffer; Substrate; Developer; Enzyme Mix (200μl); Cysteine; (NH4)2Fe(SO4)2; Isocitrate Standard (10μmol)
• Target Species: Mammalian
• Detection method: Absorbance at 450 nm
• Features & Benefits: Simple procedure; takes ~ less than 40 minutes; Fast and convenient