Active GMP Recombinant Human GDNF Protein

• Cat.No.: GDNF-133HG
• Product Overview: Recombinant Human GDNF, Arg109-Ile211, was produced in NS0 cell under cGMP guidelines.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Protein Length: 109-211 a.a.
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
  The specific activity of recombinant human GDNF is approximately 3.1 x 10^3 units/μg, which is calibrated against recombinant human GDNF Reference Standard (NIBSC code: 09/266).
  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
• Molecular Mass: Predicted Molecular Mass: 11.6 (monomer) kDa
• N-terminal Sequence Analysis: Arg109-Gly-Gln-Arg-Gly-Lys-Asn-Arg-Gly-(Cys)
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: GDNF glial cell derived neurotrophic factor [ Homo sapiens ]
• Official Symbol: GDNF
• Synonyms: GDNF; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF
• Gene ID: 2668
• mRNA Refseq: NM_000514
• Protein Refseq: NP_000505
• MIM: 600837
• UniProt ID: P39905