Active GMP Recombinant Human IL12 Protein
|Product Overview :||Recombinant Human IL12 (Arg23-Ser219&Ile23-Ser328) was produced in HEK293 cell in an animal component free process under cGMP guidelines.|
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|Description :||IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.|
|Source :||HEK293 cell|
|Bio-activity :||Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL).
The ED50 for this effect is typically 0.5 ng/ml.
|AA Sequence :||RNLPVATPDPGMFPCLHHSQNLLRA VSNMLQKARQTLEFYPCTSEEIDHE DITKDKTSTVEACLP LELTKNESCLNSRETSFITNGSCLA SRKTSFMMALCLSSIYEDLKMYQVE FKTMNAKLLMDPKRQ IFLDQNMLAVIDELMQALNFNSETV PQKSSLEEPDFYKTKIKLCILLHAF RIRAVTIDRVMSYLN AS&IWELKKDVYVVELDWYPDAPGEMVV LTCDTPEEDGITWTLDQSSEVLGSG KTLTIQVKEFGD AGQYTCHKGGEVLSHSLLLLHKKED GIWSTDILKDQKEPKNKTFLRCEAK NYSGRFTCWWLTTIS TDLTFSVKSSRGSSDPQGVTCGAAT LSAERVRGDNKEYEYSVECQEDSAC PAAEESLPIEVMVDA VHKLKYENYTSSFFIRDIIKPDPPK NLQLKPLKNSRQVEVSWEYPDTWST PHSYFSLTFCVQVQG KSKREKKDRVFTDKTSATVICRKNA SISVRAQDRYYSSSWSEWASVPCS|
|Residual Host Cell DNA Content :||<10pg/mg|
|Residual Host Cell Protein Content :||<1ug/mg|
|Purity :||Greater than 95% as determined by reducing SDS-PAGE.|
|Usage :||Recommemnd working concentration: 0.5ng/ml|
|Storage :||Lyophilized and reconstituted protein should be stored at -80 centigrade. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.|
|Reconstitution :||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration >100μg/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
|Quality Statement :||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name :||IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ]
IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens
|Synonyms :||IL-12A; NFSK; P35; IL-12 subunit p35; IL-12, subunit p35; IL12A; IL12B; NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; p40; IL 12B; IL12; subunit p40; interleukin 12; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin|
|Gene ID :||3592;
|mRNA Refseq :||NM_000882;
|Protein Refseq :||NP_000873;
|UniProt ID :||P29459;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Fluorescence microscopy tracks IL12 internalization and trafficking.
Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.
Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.
Reporter gene assays analyze IL12's promoter activity and response elements.
Super-resolution imaging details IL12's cellular distribution and dynamics.
3D cell culture models reveal IL12's effects on tissue microenvironments.
Co-localization studies and co-IP assays identify IL12's binding partners.
Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.
ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.
Transgenic models elucidate IL12's roles in immune response regulation.
Customer Reviews (3)Write a review
Effective in Th1 cell differentiation. -
High specificity in ELISA. -
Stable under storage. -
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