Active GMP Recombinant Human IL12B Protein

• Cat.No.: IL12B-128HG
• Product Overview: Recombinant Human IL12B(Ile23–Ser328) and Human IL12A(Arg23–Ser219), was co-produced in Sf 21 (baculovirus)-derived under cGMP guidelines.

Specification

• Source: Insect cell
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
• Molecular Mass: Predicted Molecular Mass: 34.7 (p40) & 22.5 (p35) kDa
  SDS-PAGE: 41 kDa (p40) and 29 kDa (p35), reducing conditions
• N-terminal Sequence Analysis: Ile-Trp-Glu-Leu-Lys-Lys-Asp-Val-Tyr-Val (p40)
  Arg-Asn-Leu-Pro-Val-Ala-Thr-Pro-Asp-Pro (p35)
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
• Tag: Non
• Protein length: Ile23-Ser328;Arg23-Ser219

Gene Information

• Gene Name: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
• Official Symbol: IL12B
• Synonyms: IL12B; NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; IL-12B
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• MIM: 161561
• UniProt ID: P29460

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.