Active GMP Recombinant Human IL18 Protein
Cat.No. : | IL18-155HG |
Product Overview : | Recombinant human IL18 (NP_001553.1) was expressed in E. coli. |
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Source : | E. coli |
Species : | Human |
Tag : | Non |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS, 0.02 % Tween-20, pH 7.0. |
Bio-activity : | Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 1.5 - 15 ng/mL. The specific activity of recombinant human IL-18 GMP is ≥ 3.0 × 106 IU/mg, which is calibrated against the human IL-18 reference standard (NIBSC code: 03/200). |
Molecular Mass : | Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids. |
Protein Length : | 157aa |
AA Sequence : | YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIIS MYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDII FFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIM FTVQNED |
Endotoxin : | Less than 0.01 EU/μg of rHuIL-18 GMP as determined by LAL method. |
Purity : | > 95 % by SDS-PAGE and > 98 % by HPLC analyses. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1 - 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ] |
Official Symbol : | IL18 |
Synonyms : | IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g; MGC12320 |
Gene ID : | 3606 |
mRNA Refseq : | NM_001243211 |
Protein Refseq : | NP_001230140 |
MIM : | 600953 |
UniProt ID : | Q14116 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewEffective in inflammation models.
High stability in serum.
Suitable for ELISA.
Q&As (10)
Ask a questionCo-immunoprecipitation, co-localization studies, and pathway inhibition assays decipher IL18's interactions with signaling molecules and pathways.
Therapeutic antibody trials, in vitro drug screens, and disease model assessments assess IL18-targeted interventions in autoimmune diseases.
Xenograft models, intravital imaging, and tissue-specific knockout approaches unravel IL18's kinetics and effects within complex in vivo environments.
Flow cytometry, chemotaxis assays, and functional cell assays examine IL18's impact on immune cell activation, migration, and effector functions.
Surface plasmon resonance, mutational studies, and phosphoproteomics illuminate IL18's receptor interactions and downstream signal transduction pathways.
Immunophenotyping, cytokine profiling, and gene expression analyses elucidate IL18's influence on Th cell polarization and immune balance.
ChIP assays, promoter analyses, and epigenetic profiling uncover the regulatory mechanisms underlying IL18 gene expression dynamics.
Disease models, knockout mice, and co-stimulation studies explore IL18's involvement in inflammatory conditions and its interplay with other proinflammatory mediators.
Tumor models, immune profiling, and immunotherapeutic studies investigate IL18's modulation of the tumor microenvironment and its implications for cancer therapy.
Caspase assays, cytokine ELISAs, and microscopy techniques unveil the activation of inflammasomes and IL18's contribution to pyroptosis and cytokine storms.
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