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Active GMP Recombinant Human IL18 Protein

Cat.No. : IL18-155HG
Product Overview : Recombinant human IL18 (NP_001553.1) was expressed in E. coli.
  • Specification
  • Gene Information
  • Related Products
Source : E. coli
Species : Human
Tag : N/A
Form : Lyophilized from a 0.2 μm filtered solution in PBS, 0.02 % Tween-20, pH 7.0.
Bio-activity : Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 1.5 - 15 ng/mL. The specific activity of recombinant human IL-18 GMP is ≥ 3.0 × 106 IU/mg, which is calibrated against the human IL-18 reference standard (NIBSC code: 03/200).
Molecular Mass : Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
Protein Length : 157aa
AA Sequence : YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIIS MYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDII FFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIM FTVQNED
Endotoxin : Less than 0.01 EU/μg of rHuIL-18 GMP as determined by LAL method.
Purity : > 95 % by SDS-PAGE and > 98 % by HPLC analyses.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1 - 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃. Further dilutions should be made in appropriate buffered solutions.
Gene Name : IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
Official Symbol : IL18
Synonyms : IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g; MGC12320
Gene ID : 3606
mRNA Refseq : NM_001243211
Protein Refseq : NP_001230140
MIM : 600953
UniProt ID : Q14116

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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