| Species : |
Human |
| Source : |
E. coli |
| Tag : |
Non |
| Form : |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 3.5. |
| Bio-activity : |
Fully biologically active when compared to standard. Measured in a cell proliferation assay using CTLL-2 cells. The specific activity of recombinant human IL-2 is > 1.0 × 10^7 IU/mg, which is calibrated against rHuIL-2 WHO International Standard (NIBSC code: 86/500). |
| Molecular Mass : |
Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids. |
| AASequence : |
APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFCQSIIS TLT |
| Endotoxin : |
Less than 0.01 EU/µg of rHuIL-2 GMP as determined by LAL method. |
| Purity : |
> 98 % by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
