Active GMP Recombinant Human IL36A protein

Cat.No. : IL36A-4338HG
Product Overview : Recombinant Human IL36A was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.
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Description : Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant human interleukin-36 alpha contains 158 amino acids residues which is a single non-glycosylated polypeptide and it is 30 % a.a. identical to IL-1ra, and 27 %, 31 %, 36 %, 46 %, 57% and 28 % a.a. identical to IL-1β, IL-36Ra/IL-1F5, IL-37/IL-1F7, IL-36β/IL-1F8, IL-36γ/IL-1F9 and IL-1F10.
Source : E. coli
Species : Human
Form : Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36α at 1 µg/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 µg/mL.
Molecular Mass : Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.
AA Sequence : MEKALKIDTPQQGSIQDINHRVWVL QDQTLIAVPRKDRMSPVTIALISCR HVETLEKDRGNPIYLGLNGLNLCLM CAKVGDQPTLQLKEKDIMDLYNQPE PVKSFLFYHSQSGRNSTFESVAFPG WFIAVSSEGGCPLILTQELGKANTT DFGLTMLF
Endotoxin : Less than 1 EU/μg of rHuIL-36α, 158a.a. as determined by LAL method.
Purity : > 95 % by SDS-PAGE and HPLC analyses.
Storage : This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably des centigradecated. Upon reconsitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw centigrade centigradeles.
Reconstitution : Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Quality Statement : Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name : IL36A interleukin 36, alpha [ Homo sapiens (human) ]
Official Symbol : IL36A
Synonyms : FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
Gene ID : 27179
mRNA Refseq : NM_014440.2
Protein Refseq : NP_055255.1
MIM : 605509
UniProt ID : Q9UHA7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does IL36A carry a fever risk? 04/27/2022

In general, the use of IL36A does not cause significant fever reactions, but individual differences exist, if there are abnormalities, please consult a doctor.

How to prepare IL36A? 09/07/2021

The IL36A is expressed, purified, and constructed using recombinant DNA technology in a suitable host expression system, usually through genetic engineering.

In what inflammatory diseases does recombinant IL36A have potential applications? 05/24/2021

IL36A has potential application value in psoriasis, arthritis and other inflammatory diseases, but the specific efficacy needs further study.

Does IL36A have anti-tumor effects? 08/22/2020

IL36A has been shown in some studies to have anti-proliferative and anti-invasive effects on certain tumors, but more studies are needed to confirm its specific mechanism of action and effect.

How is the research progress of IL36A? 06/05/2020

The research of IL36A is progressing continuously, and some studies have shown its potential in immune regulation, anti-inflammatory effect and so on.

Can IL36A be used in animal experiments? 02/13/2019

Yes, IL36A can be used in animal studies to investigate its mechanism of action and potential applications.

Customer Reviews (3)

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Reviews
07/20/2022

    Western blot can simultaneously detect the purity and specificity of IL-36A. -

    03/12/2021

      The purity of IL-36A can be preliminarily detected by SDS-PAGE electrophoresis and verified and quantified according to molecular weight. -

      08/12/2020

        Infrared spectroscopy can be used to detect the structural characteristics of IL-36A, such as the main structure of the protein and the interaction of amino acid residues. -

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