Active GMP Recombinant Human IL36A protein
|Product Overview :||Recombinant Human IL36A was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
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|Description :||Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36α, IL-36β, and IL-36γ (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. Studies showed IL-36α is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. Recombinant human interleukin-36 alpha contains 158 amino acids residues which is a single non-glycosylated polypeptide and it is 30 % a.a. identical to IL-1ra, and 27 %, 31 %, 36 %, 46 %, 57% and 28 % a.a. identical to IL-1β, IL-36Ra/IL-1F5, IL-37/IL-1F7, IL-36β/IL-1F8, IL-36γ/IL-1F9 and IL-1F10.|
|Source :||E. coli|
|Form :||Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.|
|Bio-activity :||Fully biologically active when compared to standard. The specific activity determined by its ability in a functional ELISA. Immobilized rHuIL-36α at 1 µg/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 µg/mL.|
|Molecular Mass :||Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.|
|AA Sequence :||MEKALKIDTPQQGSIQDINHRVWVL QDQTLIAVPRKDRMSPVTIALISCR HVETLEKDRGNPIYLGLNGLNLCLM CAKVGDQPTLQLKEKDIMDLYNQPE PVKSFLFYHSQSGRNSTFESVAFPG WFIAVSSEGGCPLILTQELGKANTT DFGLTMLF|
|Endotoxin :||Less than 1 EU/μg of rHuIL-36α, 158a.a. as determined by LAL method.|
|Purity :||> 95 % by SDS-PAGE and HPLC analyses.|
|Storage :||This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably des centigradecated. Upon reconsitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw centigrade centigradeles.|
|Reconstitution :||Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.|
|Quality Statement :||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name :||IL36A interleukin 36, alpha [ Homo sapiens (human) ]|
|Official Symbol :||IL36A|
|Synonyms :||FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)|
|Gene ID :||27179|
|mRNA Refseq :||NM_014440.2|
|Protein Refseq :||NP_055255.1|
|UniProt ID :||Q9UHA7|
|IL36A-5238HCL||Recombinant Human IL1F6 293 Cell Lysate||+Inquiry|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (6)Ask a question
In general, the use of IL36A does not cause significant fever reactions, but individual differences exist, if there are abnormalities, please consult a doctor.
The IL36A is expressed, purified, and constructed using recombinant DNA technology in a suitable host expression system, usually through genetic engineering.
IL36A has potential application value in psoriasis, arthritis and other inflammatory diseases, but the specific efficacy needs further study.
IL36A has been shown in some studies to have anti-proliferative and anti-invasive effects on certain tumors, but more studies are needed to confirm its specific mechanism of action and effect.
The research of IL36A is progressing continuously, and some studies have shown its potential in immune regulation, anti-inflammatory effect and so on.
Yes, IL36A can be used in animal studies to investigate its mechanism of action and potential applications.
Customer Reviews (3)Write a review
Western blot can simultaneously detect the purity and specificity of IL-36A. -
The purity of IL-36A can be preliminarily detected by SDS-PAGE electrophoresis and verified and quantified according to molecular weight. -
Infrared spectroscopy can be used to detect the structural characteristics of IL-36A, such as the main structure of the protein and the interaction of amino acid residues. -
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