Active GMP Recombinant Human SIRPA protein

• Cat.No.: SIRPA-475HG
• Product Overview: GMP Recombinant Human SIRPA protein(NP_542970.1)(241 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: HEK293
• Protein Length: DNA sequence encoding Human SIRP alpha(NCBI Reference Sequence: NP_542970.1) was expressed with His tag at the C-terminal.
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: The linear range of immobilized Human CD47(Fc Tag) at 5µg/mL(100 uL/well) that can bind to human SIRP alpha (His Tag) is 10-500ng/mL.
• Molecular Mass: Recombinant Human SIRP alpha protein contains 241 aa. It has a predicted MW of 39 kDa.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SIRPA signal-regulatory protein alpha [ Homo sapiens ]
• Official Symbol: SIRPA
• Synonyms: SHPS1; SIRPA; CD172A; BIT; MFR; MYD1; P84; PTPNS1
• Gene ID: 140885
• mRNA Refseq: NM_001040022
• Protein Refseq: NP_001035111
• MIM: 602461
• UniProt ID: P78324