Active GMP Recombinant Human TNF Protein

• Cat.No.: TNF-106HG
• Product Overview: Recombinant Human TNF, Val77-Leu233, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 77-233 a.a.
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
  The specific activity of recombinant human TNF-alpha GMP is approximately 7.6 x 10^4 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786).
• Molecular Mass: Predicted Molecular Mass: 17 kDa
  SDS-PAGE: 17 kDa, reducing conditions
• N-terminal Sequence Analysis: Met-Val77-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100-200 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; DIF; TNF superfamily; member 2; TNF alpha; TNFSF2; TNF-a; cachectin; tumor necrosis factor-alpha; TNFA; TNF-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375