Active GMP Recombinant Serratia Nuclease

• Cat.No.: N-5633B
• Product Overview: GMP Recombinant Serratia Nuclease was expressed in E. coli.

Specification

• Species: Serratia
• Source: E.coli
• Tag: Non
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in 50 mM Tris-HCl, 30 mM NaCl, 2 mM MgSO4, 2 mM DTT, 50% Glycerol, pH 8.0.
• Bio-activity: Test by degradation of Salmon Sperm DNA, corresponding to a specific activity of > 1.1 × 10^6 U/mg.
• Molecular Mass: Approximately 26.8 kDa, a single non-glycosylated polypeptide chain containing 245 amino acids.
• Endotoxin: Less than 0.25 EU/KU of BenzoNuclease GMP as determined by LAL method.
• Purity: > 99% by SDS-PAGE analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.