Active MSLN protein-coupled magnetic Beads

Species :	Human
Source :	HEK293
Protein Length :	Glu 296 - Gly 580 (R309P)
Appearance :	Powder mixture
Particle size :	2 μm
Bio-activity :	Immobilized 19.5 μg MSLN to 1mg Beads can bind the Anti MSLN Ab with an EC50 of 0.1037 μg/mL.
Coupled amount of protein :	≈330 pmol (19.5μg) Mesothelin / mg beads
Capacity :	>270 pmol (16 ug) Anti-hMSLN antibody / mg beads
Formulation :	PBS, pH7.4, with 10% Trehalose
Reconstitution :	2 mL sterile deionized water (1 mg beads/mL)
Background :	The Human Mesothelin-coupled Magnetic Beads is produced by coupling biotinylated mesothelin protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-mesothelin antibody from your sample with high specificity.
Application :	This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
Storage :	Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.