Active Native Bovine Collagen Type V Protein

• Cat.No.: Col-01B
• Product Overview: Bovine COLLAGEN Type V has been prepared from Bovine Placenta and is chromatographically and immunologically pure.

Specification

• Description: In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6% of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Type IV collagen is a major macromolecular constituent of basement membranes and can be readily isolated from basement-membrane-rich tissues or highly vascularized tissues such as the placental villi. This collagen appears to be largely restricted to structures identifiable as basement membranes. In contrast, type VI collagen appears to be prevalent in several tissues even though it has been isolated largely from placental villi preparations. The extent to which type VII and VIII collagens are distributed is not known.
• Source: Bovine
• Species: Bovine
• Form: Liquid (sterile filtered). 0.5M Acetic Acid.
• Bio-activity: This product reacts with anti-Collagen Type V. Reaction with anti-Collagen I, II, III, IV or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
• Purity: Bovine COLLAGEN Type V has been prepared from Bovine Placenta and is chromatographically and immunologically pure.
• Applications: ELISA: 1:10,000; Western Blot: 1:1000; Immunohistochemistry: 1:100-1:500; Other Dilution: User Optimized. For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.
• Storage: Store vial at 4 centigrade prior to opening. This product is stable at 4 centigrade as an undiluted liquid. Dilute only prior to immediate use.
• Concentration: 1 mg/ml by UV absorbance at 280 nm.
• Preservative: 0.01% (w/v) Sodium Azide

Gene Information

• Gene Name: COL5A1
• Official Symbol: COL5A1
• Synonyms: Extracellular Matrix Protein; EDSC; EDSCL1
• Gene ID: 100848491
• mRNA Refseq: XM_024999726.1
• Protein Refseq: XP_024855494.1
• UniProt ID: G3MZI7