Active Native Bovine FGF2 Protein

• Cat.No.: FGF2-40B
• Product Overview: Native Bovine FGF2 Protein is purified from Bovine brain tissue.

Specification

• Source: Bovine brain
• Species: Bovine
• Form: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 0.125-0.625 ng/mL.
• Molecular Mass: 18 kDa
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: fibroblast growth factor 2 [ Bos taurus (cattle) ]
• Official Symbol: FGF2
• Synonyms: BFGF; FGF-2; HBGF-2
• Gene ID: 281161
• mRNA Refseq: NM_174056
• Protein Refseq: NP_776481
• UniProt ID: F1N3T9