| Species : |
Datura stramonium |
| Source : |
Datura stramonium |
| Tag : |
Non |
| Description : |
DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein. The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. |
| Bio-activity : |
Inhibiting/Eluting Sugar: Chitin Hydrolysate |
| Molecular Mass : |
86 Kda |
| Applications : |
Glycobiology, Mitogenic Stimulation |
| Usage : |
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM Ca2+ is recommended. For preserving solutions stored at 4 centigrade, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml. |
| Storage : |
Refrigerate; aliquots of reconstituted lectin can be stored frozen. or preserved with 0.08% sodium azide and stored at 4 centigrade |
