Active Native Human Thrombin Protein

• Cat.No.: Thrombin-22H
• Product Overview: Native Human Thrombin was purified from Human Plasma.

Specification

• Species: Human
• Source: Human Plasma
• Tag: Non
• Form: Lyophilized from 20 mM bis-tris, 150 mM NaCl, 0.1% PEG 8000, pH 6.5.
• Bio-activity: > 2,800 NIH U/mg protein
• Molecular Mass: 36 kDa
• Purity: > 95% (SDS-PAGE)
• Applications: Diagnostic Controls, Calibrators & Standards; Immunoassays; Antibody Production; Clinical Chemistry; Testing/Assay Validation; Life Science; ELISA, Blotting, Etc.; Manufacturing; Coagulation/Anemia
• Storage: 2-8 centigrade
• Reconstitution: A working stock solution is prepared by adding 1 mL buffered saline, pH 7.4 (ex. 25mM HEPES, 150mM sodium chloride, 0.1% PEG 8000, pH 7.4).
  Further dilution should be made in buffer containing a suitable blocking agent such as 0.1%-1% Prionex, BSA or PEG.
• Publications:
  Comparison of mechanical compressive properties of commercial and autologous fibrin glues for tissue engineering applications (2017)

Citation

Comparison of mechanical compressive properties of commercial and autologous fibrin glues for tissue engineering applications.

Journal: Clinical biomechanics (Bristol, Avon)    PubMed ID: 28863319    Data: 2018/4/27

Authors: Matthew G Cravens, Anthony W Behn, Jason L Dragoo

Article Snippet:An Emcyte Executive Series centrifuge (Emcyte Corporation, Fort Myers, FL, USA) and a Pure PRP system (Biorich Medical, Irvine, CA, USA) were used to produce PPP.An Emcyte Executive Series centrifuge (Emcyte Corporation, Fort Myers, FL, USA) and a Pure PRP system (Biorich Medical, Irvine, CA, USA) were used to produce PPP.. The ProPlaz product was then used to remove water from the PPP by pushing the sample repetitively across a filter, until the PPP was reduced to 2/3 its original volume, resulting in 10 mL of “PPP concentrate.” Each autologous product was then gelled by combining 500 IU human thrombin (Thrombin 22-H, Creative BioMart, Shirley, NY, USA) in 40 mM CaCl2 solution.. For both Angel and ProPlaz, an Evicel applicator was used to draw PPP into a 10 mL syringe, which was then combined with 1 mL of thrombin solution to make the autologous fibrin gel.For both Angel and ProPlaz, an Evicel applicator was used to draw PPP into a 10 mL syringe, which was then combined with 1 mL of thrombin solution to make the autologous fibrin gel.