Active Native Human Thyroid Stimulating Hormone

• Cat.No.: TSH-108H
• Product Overview: Human Thyroid Stimulating Hormone was purified from Human pituitary glands.

Specification

• Description: Thyroid-stimulating hormone is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3). TSH production is controlled by a Thyrotropin Releasing Hormone, (TRH), which is manufactured in the hypothalamus and transported to the Anterior Pituitary gland, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release. The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH, When the levels of T3 and T4 are low , the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit. The a (alpha) subunit is identical to that of human chorionic gonadotropin (HCG), luteinising hormone (LH), follicle-stimulating hormone (FSH). The b (beta) subunit is unique to TSH, and therefore determines its function .
• Source: Human pituitary glands
• Species: Human
• Form: Lyophilized from a concentrated (1.31mg/1ml) solution containing 50mM ammonium bicarbonate.
• Bio-activity: 9.3 IU/mg vial by Centaur CP. Siemens Centaur CP is standardized against WHO 3rd IRP 81/565.
• Purity: ≥95% as determined by SDS PAGE
• Storage: Lyophilized should be stored desiccated below -18°C. Upon reconstitution TSH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Tag: Non

Reviews

07/11/2022

Good for Bimo-lecular fluorescence complementation control.

06/01/2020

TSH manufacturer's products are consistently of the highest quality, with a strong commitment to purity, potency, and consistency.

05/17/2020

Work great in Western blot analysis.

12/02/2019

The purity is truly exceptional, with no visible impurities or contaminants.

03/18/2019

TSH is ideal for Western-blot analysis, with strong bands and clear specificity.

04/09/2018

Can save materials and shorten the experiment time in luci-ferase complementation assay.

04/03/2018

It was accurately quantified in expression analysis.

03/11/2017

It is appropriate for T cell killing analysis.

Q&As

What the TSHR signal transduction, regulation, and maturation?

The TSHR, long known to signal via cAMP, can induce both the phospholipase C (PLC) and the protein kinase A signal transduction systems. Intracellular Ca2+ and PLC regulate iodide efflux, H2O2 production, and thyroglobulin iodination, while adenylate cyclase and cAMP regulate iodide uptake and transcription of thyroglobulin (Tg), thyroid peroxidase (TPO), and the sodium-iodide symporter.

Why the puzzling size diversity of TSHRs detected by western blotting?

Presumably by protein disulfide isomerase leading to release of the α subunit from the membrane-bound receptor.

What is associated with a high TSH value?

High-normal TSH-values are associated with an adverse lipid profile, high blood pressure, high body mass index (BMI), metabolic syndrome and fatal coronary heart disease.

How is TSH level regulated in brain?

It is known that the majority of the metabolically active T3 in the brain is not derived from the circulation, but produced locally by the effect of D2 on T4. Locally produced T3 largely acts on the hypothalamus and pituitary glands to control TSH levels.

Are TSH receptors expressed in places other than the thyroid gland?

Thyroid stimulating hormone receptors have been identified in mammalian heart, bone, kidneys, brain, skin, adipose, hair follicles, thymus, lymphocytes, astrocyte cultures and in fish testes.

How can thyroid cancer be treated by inhibiting thyrotropin?

Levothyroxine is recommended for long-term TSH suppression.

What cells or organs does TSH work on?

Including thyroid,lymphocytes, the pituitary, thymus, testes, kidney, brain, adipose tissue, and fibroblasts.

What is the biological function of thyroid stimulating hormone (TSH)?

The only established biologic function of thyroid stimulating hormone (TSH) is to regulate the synthesis and secretion of thyroid hormone from thyroid follicular cells.

How to achieve TSH transcription?

In response to low circulating thyroid hormone levels, the basal medial hypothalamus releases thyrotropin-releasing hormone (TRH) which then stimulates production of thyroid-stimulating hormone (TSH) from the thyrotropes of the anterior pituitary gland.

Besides TSH, what other substances can stimulate the release of prolactin?

Other substances like serotonin, gonadotropin-releasing hormone, and estrogen can also stimulate prolactin release.

What is the role of TSH protein in thyroid cancer?

In thyroid cancer, the TSH protein may have a role in promoting the proliferation and growth of cancer cells. Therefore, for some high-risk groups, such as patients with postoperative thyroidectomy, inhibition of TSH protein production and activity can reduce the risk of cancer recurrence.

What factors regulate the secretion of TSH protein?

The secretion of TSH protein is regulated by a variety of factors, including negative feedback regulation of the hypothalamic-pituitary-thyroid axis, thyroxine levels, and the regulatory mechanism of anterior pituitary cells.

Does the TSH protein have polymorphisms?

The polymorphisms of the TSH protein are partly related to the genetic variation of individuals. TSH proteins of different genotypes may affect an individual's sensitivity to TSH and regulation of thyroid function.

How does TSH protein regulate thyroid function?

TSH protein activates intracellular signaling pathways by binding to TSH receptors on the surface of thyroid cells, promoting thyroxine synthesis and secretion. It enhances the production of thyroxine by positively regulating iodine uptake, thyroglobulin synthesis, and thyroid peroxidase activity.

What are the applications of TSH protein in clinical diagnosis?

The TSH protein plays a key role in clinical diagnosis. By measuring serum TSH levels, it is possible to assess whether the thyroid gland is functioning normally for diagnosis and follow-up of thyroid-related diseases.

Does the TSH protein interact with other proteins?

Yes, the TSH protein can interact with TSH receptors on the surface of thyroid cells, triggering a series of signaling. In addition, TSH protein interacts with other cytokines and regulators to regulate thyroid function.