Recombinant Aequorea victoria GFP protein, His-tagged

• Cat.No.: GFP-03A
• Product Overview: Recombinant Aequorea victoria GFP protein(AAB65663) (Ser 2-Lys 238) was expressed in E.coli, with a polyhistide tag at the N-terminus.

Specification

• Species: Aequorea victoria
• Source: E.coli
• Tag: His
• Protein Length: 2-238 aa
• Form: Lyophilized from sterile PBS, pH 7.5.
• Molecular Mass: The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7 kDa. It migrates as an 35 kDa band in SDS-PAGE under reducing conditions as predicted.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Evolution of structure-function relationships in the GFP-family of proteins. (2014)

Gene Information

• Official Symbol: GFP