Active Recombinant B. thetaiotaomicron BT_4675 Protein, Met & His-tagged

• Cat.No.: BT_4675-863B
• Product Overview: Recombinant B. thetaiotaomicron BT_4675 (Accession # NP_813586) Met1-Arg376, fused with Met, 6-His tag and a fusion partner at the N-terminus, was produced in E. coli.

Specification

• Species: B.thetaiotaomicron
• Source: E.coli
• Tag: His
• Protein Length: Met1-Arg376
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Citrate.
• Bio-activity: Measured by its ability to liberate oligosaccharides from heparin.The specific activity is >12,000 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
• Molecular Mass: Recombinant B. thetaiotaomicron BT_4675, Met & His-tagged has a calculated MW of 55 kDa. In SDS-PAGE migrates as 59 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: BT_4675 heparin lyase I [ Bacteroides thetaiotaomicron VPI-5482 ]
• Official Symbol: BT_4675
• Synonyms: BT_4675
• Gene ID: 1.0734e+006
• Protein Refseq: NP_813586.1