Active Recombinant B. thetaiotaomicron OGA, His-tagged

• Cat.No.: OGA-85B
• Product Overview: Recombinant B. thetaiotaomicron OGA(Gln22-Lys737), fused with an N-terminal Met and 6-His tag, was expressed in E. coli.

Specification

• Description: Protein O-GlcNAcase (EC 3.2.1.169, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme with system name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase.
• Source: E. coli
• Species: B. thetaiotaomicron
• Tag: His
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij and Glycerol.
• Bio-activity: Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-beta -D-glucosaminide (4-MU-GlcNAc) The specific activity is >3500 pmol/min/µg.
• Molecular Mass: Predicted Molecular Mass:83 kDaSDS-PAGE:66-76 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 ºC as supplied.3 months, -20 to -70 ºC under sterile conditions after opening.