Active Recombinant B. thetaiotaomicron OGA, His-tagged

Cat.No. : OGA-85B
Product Overview : Recombinant B. thetaiotaomicron OGA(Gln22-Lys737), fused with an N-terminal Met and 6-His tag, was expressed in E. coli.
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Description : Protein O-GlcNAcase (EC, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme with system name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase.
Source : E. coli
Species : B. thetaiotaomicron
Tag : His
Predicted N Terminal : Met
Form : Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij and Glycerol.
Bio-activity : Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-beta -D-glucosaminide (4-MU-GlcNAc) The specific activity is >3500 pmol/min/µg.
Molecular Mass : Predicted Molecular Mass:83 kDaSDS-PAGE:66-76 kDa, reducing conditions
Endotoxin : < 1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
Storage : Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 ºC as supplied.3 months, -20 to -70 ºC under sterile conditions after opening.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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