Active Recombinant Bacteriophage T4 Polynucleotide Kinase

• Cat.No.: pseT-58B
• Product Overview: Purified from a strain of?E.coli?that expresses the recombinant T4 Polynucleotide Kinase gene.

Specification

• Species: Bacteriophage T4
• Source: E.coli
• Tag: Non
• Description: T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules. T4 PNK also exhibits 3′-phosphatase and 2′, 3′ cyclicphosphodiesterase activities.
• Bio-activity: 133,333 U/mg
• Molecular Mass: 34,620 Daltons
• Purity: >99% by SDS-PAGE
• Unit Definition: 1 unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer.
• Storage: -25 to -15°C
• Concentration: 10,000 U/m
• Storage Buffer: Supplied in: 10mM Tris-HCL, 50 mM KCl, 0.1 μM ATP, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.4 @ 25?C. Supplied with: 10X T4 Polynucleotide Kinase Buffer: 700mM Tris-HCl, 100mM MgCl2, 50mM DTT pH 7.6 @ 25?C.

Gene Information

• Gene Name: pseT?polynucleotide kinase [?Enterobacteria phage T4?]
• Official Symbol: pseT
• Synonyms: pseT; polynucleotide kinase; NP_049834.1; 5"-hydroxyl kinase, a 3"-phosphatase and a 2",3"-cyclic phosphodiesterase; catalyzes the transfer of the terminal phosphate of ATP to the 5"-hydroxyl termini of ribo- and deoxyribonucleotides; in the presence of ADP the enzyme also catalyzes the exchange of the 5" terminal phosphate from phosphorylated DNA to ADP; these activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase
• Gene ID: 1258796
• Protein Refseq: NP_049834
• UniProt ID: 2IA5_D