Active Recombinant BK (SVTLE)

• Cat.No.: BK(SVTLE)-649
• Product Overview: Recombinant BK (SVTLE) produced in E. coli is a single, glycosylated, polypeptide chain containing 231 amino acids, and having a molecular mass of approximately 28,000～33,000 Dalton.

Specification

• Description: Batroxobin is a serine protease derived from the venom of Bothrops atrox. This thrombin-like proteolytic enzyme splits the 16 Arg-17 Gly bond in the A (alpha)-chain of fibrinogen, releasing fibrinopeptide A and leading to the clot formation through aggregation of ormed of fibrin I monomer or Des-AA-monomer. In contrast to other anti-coagulants, it does not affect the functions of platelets. Hence, Batroxobin is used to determine fibrinogen in plasma, to measure a "batroxobin clotting time" as a heparin-insensitive parallel to the thrombin time, to investigate dysfibrinogenemia, and to test the contractile system of platelets.
• Source: Pichia pastoris
• Form: Lyophilized from a 0.2 μm filtered concentrated sodium acetate buffer solution.
• Bio-activity: Fully biologically active when compared to standard. The biologically activity of rRpBX is no less than 500 KU/mg (Klobusitzky Unit). One KU is defined as the amount of enzyme which coagulates standard human plasma incubated at 37 centigrade in vitro wit
• Molecular Mass: 28,000～33,000 Dalton
• AA Sequence: VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIH LGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSE HIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVC REAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCA EPRKPAFYTK VFDYLPWIQS IIAGNKTATC P
• Endotoxin: Less than 0.5 EU/μg of rRpBX/SVTLE as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apport
• Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportion