Active Recombinant Bovine Enterokinase, His-tagged

• Cat.No.: Enterokinase-11082B
• Product Overview: Enterokinase is expressed from Pichia Pastoris, it contained the light chain of the bovine enterokinase. Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. This protease with 10 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.

Specification

• Species: Bovine
• Source: Yeast
• Tag: His
• Description: Enterokinase is expressed from Bovine Yeast.
• Form: Supplied as 0.2 μm filtered solution in 20 mM Tris, 200 mM NaCl, pH7.2 with glycerol as protectant.
• Bio-activity: Enzyme Activity: >8 U/μL Unit Definition: 1 unit is defined as the amount of enzyme required to cleave 25 μg of a MBP-EK-paramyosin-ΔSal substrate to 95% completion in 16 hours at 25 centigrade in a total reaction volume of 100 μL.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Applications: Protein Purification. Fusion Protein Cleavage.
• Storage: This product is stable after storage at: The product MUST be stored at -20 centigrade or lower upon receipt; -20 centigrade for 3 months under sterile conditions.
• Concentration: 0.2 mg/mL
• Shipping: This product is supplied and shipped with dry ice, please inquire the shipping cost.

Gene Information

• Official Symbol: Enterokinase
• Synonyms: Enterokinase