Active Recombinant Canavalia ensiformis α1-2,3,6 Mannosidase

• Cat.No.: Mannosidase-10C
• Product Overview: Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities

Specification

• Species: Canavalia ensiformis
• Source: Pichia pastoris
• Description: α1-2,3,6 Mannosidase is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing α1-2, α1-3 and α1-6 linked mannose residues from oligosaccharides.
• Molecular Mass: 110 kDa
• Purity: ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
• Reaction conditions: 1× GlycoBuffer 4 Supplement with 1× Zinc Incubate at 37 centigrade 1× GlycoBuffer 4: 50 mM sodium acetate (pH 4.5 at 25 centigrade)
• Unit definition: One unit is defined as the amount of enzyme required to hydrolyze more than 95% of the terminal mannose from 1 nmol of Man(α1,3)-Man(β1,4)-GlcNAc-7-amino-4-methyl-coumarin (AMC) in a total reaction volume of 10 μL at 37 centigrade within 1 hour.
• Storage Buffer: 20 mM Tris-HCl 50 mM NaCl pH 7.5 at 25 centigrade
• Heat inactivation: 95 centigrade for 10 minutes

Gene Information

• Official Symbol: α1-2,3,6 Mannosidase
• Synonyms: α1-2,3,6 Mannosidase