Active Recombinant Canine CA9 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CA9-1039CAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant canine CA9 (NP_001138646.1) (Met1-Leu410) was expressed with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Dog
• Tag: His
• Form: Lyophilized
• Bio-activity: Measured by its esterase activity. The specific activity is > 100 pmoles/min/ μg.
• Molecular Mass: The recombinant canine CA9 comprises 384 amino acids and has a predicted molecular mass of 42.1 kDa. The apparent molecular mass of the protein is approximately 50 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: 384
• N-terminal Sequence Analysis: Gln 38
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CA9 carbonic anhydrase IX [ Canis lupus familiaris ]
• Official Symbol: CA9
• Synonyms: carbonic anhydrase 9
• Gene ID: 611933
• mRNA Refseq: NM_001145174
• Protein Refseq: NP_001138646