Active Recombinant Canine CD274 protein, Fc-tagged

• Cat.No.: CD274-2132C
• Product Overview: Recombinant Canine CD274 Protein (19-238aa), was expressed in HEK293 with Fc tag.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: 19-238 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Loaded Canine PD-1, His Tag on HIS1K Biosensor, can bind Canine PD-L1, Fc Tag with an affinity constant of 85 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 51.4 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.