Active Recombinant Canine IL2 protein(Ala21-Thr155,147Cys/Ser)

• Cat.No.: IL2-445C
• Product Overview: Recombinant Canine IL2 (Q29416) (Ala 21-Thr 155, 147Cys/Ser) was expressed in E. coli, with an initial Met at the N-terminus.

Specification

• Species: Canine
• Source: E.coli
• Tag: Non
• Protein Length: Ala21-Thr155,147Cys/Ser
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant Canine IL2 at 10 μg/mL can bind recombinant human IL2Ra-Fc with a linear range of 0.3125-20 μg/ml.
• Molecular Mass: The recombinant canine IL2 consists of 136 amino acids and has a calculated molecular mass of 15.6 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 15 kDa band.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.