Active Recombinant Canine MET Protein, His-tagged, Alexa Fluor 647 conjugated
Cat.No. : | MET-1037CAF647 |
Product Overview : | Alexa Fluor 647 conjugated recombinant extracellular domain of canine MET (NP_001002963.1) (Met 1-Leu 935) was expressed, with a polyhistidine tag at the N-terminus. |
Availability | February 10, 2025 |
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Source : | HEK293 |
Species : | Dog |
Tag : | His |
Form : | Lyophilized |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human HGF at 10 μg/mL (100 μL/well) can bind canine c-MET, The EC50 of canine c-MET is 7 ng/mL. 3. Immobilized canine MET-His at 10 μg/mL (100 μL/well) can bind biotinylated human HG |
Molecular Mass : | The recombinant canine c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved α and β subunits. Each α and β subunit together consists of 922 amino acids and has a predicted molecular mass of 103 (α=33 + β=70) kDa. As a result of glycosylation, the apparent molecular mass of the canine c-Met is approximately 42-47 kDa and 85-95 kDa respectively in SDS-PAGE under reducing conditions. |
Protein length : | 992 |
N-terminal Sequence Analysis : | Glu 25 |
Endotoxin : | < 1.0 EU/ μg of the protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 647 via amines Excitation = 650 nm Emission = 668 nm |
Stability : | Samples are stable for up to 12 months from date of receipt at -70 centigrade. |
Storage : | Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Storage Buffer : | Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | MET MET proto-oncogene, receptor tyrosine kinase [ Canis lupus familiaris ] |
Official Symbol : | MET |
Synonyms : | c-Met; HGF receptor; HGF/SF receptor; SF receptor; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met |
Gene ID : | 403438 |
mRNA Refseq : | NM_001002963 |
Protein Refseq : | NP_001002963 |
UniProt ID : | Q75ZY9 |
Products Types
◆ Recombinant Protein | ||
MET-1176C | Recombinant Cynomolgus/Rhesus MET protein(Met1-Thr932) | +Inquiry |
MET-23H | Recombinant Active Human MET Protein (956-end), N-His-tagged | +Inquiry |
MET-6844H | Recombinant Human MET protein, hFc-tagged | +Inquiry |
MET-237H | Recombinant Human MET Protein (ECD), His-tagged(C-ter) | +Inquiry |
MET-2554R | Recombinant Rhesus Macaque MET Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
MET-001HCL | Recombinant Human MET cell lysate | +Inquiry |
MET-001CCL | Recombinant Cynomolgus MET cell lysate | +Inquiry |
MET-1931CCL | Recombinant Canine MET cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0550 | Met Kinase (Human) Assay/Inhibitor Screening Assay Kit | +Inquiry |
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