Active Recombinant Canine PDCD1 Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: PDCD1-1057CAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant canine PDCD1 (XP_543338.3) (Met1-Leu169) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Dog
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized canine PDCD1-Fc at 10 μg/mL (100 μL/well) can bind biotinylated canine PDCD1-Fc, The EC50 of biotinylated canine PDCD1-Fc is 0.16-0.4 μg/mL.
• Molecular Mass: 43 kDa
• Protein length: 383
• N-terminal Sequence Analysis: Leu 25
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > (20.2+76.0) % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Canis lupus familiaris ]
• Official Symbol: PDCD1
• Synonyms: PD-1; programmed death 1
• Gene ID: 486213
• mRNA Refseq: NM_001314097
• Protein Refseq: NP_001301026

Reviews

06/06/2021

Timely antibody validation, a valuable asset for our laboratory.

02/12/2021

Precise protein quantification, fundamental for maintaining data accuracy.

06/20/2019

Dependable peptide synthesis, accelerates our peptide-based projects effectively.

Q&As

What are the mechanisms by which PDCD1 modulates the immune response to viral infections?

PDCD1 modulates responses to viral infections, affecting T cell function and viral clearance.

What are the mechanisms by which PDCD1 modulates the immune response to viral infections?

PDCD1 modulates responses to viral infections, affecting T cell function and viral clearance.

What role does PDCD1 play in the development of immune tolerance?

PDCD1 helps develop immune tolerance, preventing overactive immune responses.

How does PDCD1 influence the balance between immune activation and autoimmunity?

PDCD1 maintains a balance between immune response and autoimmunity, preventing self-reactivity.

How does PDCD1 contribute to the exhaustion of T cells in chronic infections and cancer?

PDCD1 contributes to T cell exhaustion in chronic diseases and cancers, reducing immune efficacy.

How does PDCD1 influence the balance between immune activation and autoimmunity?

PDCD1 maintains a balance between immune response and autoimmunity, preventing self-reactivity.

What is the impact of PDCD1 on the effectiveness of immunotherapy in treating cancers?

PDCD1's role in immunotherapy is pivotal, as blocking PD-1 enhances anti-tumor responses.

How does PDCD1 contribute to the exhaustion of T cells in chronic infections and cancer?

PDCD1 contributes to T cell exhaustion in chronic diseases and cancers, reducing immune efficacy.

How does PDCD1 (PD-1) regulate immune checkpoint pathways in T cells?

PDCD1 regulates T cell activity by inhibiting immune responses, crucial in immune checkpoints.

What is the impact of PDCD1 on the effectiveness of immunotherapy in treating cancers?

PDCD1's role in immunotherapy is pivotal, as blocking PD-1 enhances anti-tumor responses.

What role does PDCD1 play in the development of immune tolerance?

PDCD1 helps develop immune tolerance, preventing overactive immune responses.

How do alterations in PDCD1 expression or signaling affect autoimmune disease progression?

Changes in PDCD1 signaling impact autoimmune disease progression, influencing immune system activity.

How does PDCD1 (PD-1) regulate immune checkpoint pathways in T cells?

PDCD1 regulates T cell activity by inhibiting immune responses, crucial in immune checkpoints.

How do alterations in PDCD1 expression or signaling affect autoimmune disease progression?

Changes in PDCD1 signaling impact autoimmune disease progression, influencing immune system activity.