| Species : |
Clostridium perfringens |
| Source : |
E. coli |
| Tag : |
Non |
| Description : |
α(2-3,6) Sialidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α(2-8) or α(2-9) linkages or on branched α(2-3) or α(2-6) linkages. The relative cleavage rates for different linkages are: α(2-3) > α(2-6).
α(2-3,6) Sialidase Cp will not cleave branched sialic acids (linked to an internal residue). Use α(2-3,6,8,9) Sialidase Au for α(2-8) or branched sialic acids. |
| Form : |
Sterile-filtered solution |
| EC : |
3.2.1.18 |
| Specificity : |
Cleaves all nonreducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins. |
| Contents : |
Sialidase Cp in 20 mM Tris-HCl, 25 mM NaCl, pH 7.5
5× Reaction Buffer 250 mM sodium phosphate, pH 6.0 |
| Bio-activity : |
Activity: > 15 U/mL
Specific Activity: > 250 U/mg
Defined as the amount of enzyme required to produce 1 μmole of methylumbelliferone in 1 minute at 37 centigrade, pH 5.0 from MU-NANA [2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid]. |
| Molecular Mass : |
41 kDa |
| Suggested usage : |
1. Add up to 100 μg of glycoprotein or 1 nmol of oligosaccharide to tube.
2. Add water to 14 μL
3. Add 4 μL 5× Reaction Buffer.
4. Add 2 μL α(2-3,6) Sialidase Cp.
5. Incubate at 37 centigrade for 1 hour.
Desialylation may be monitored by SDS-PAGE if the size differential between native and desialylated protein is sufficient for detection. |
| Purity : |
Neuraminidase Cp is tested for contaminating protease as follows: 10 μg of denatured BSA is incubated at 37 centigrade for 24 hours with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
The production host strain has been extensively tested and does not produce any detectable glycosidases. |
| Applications : |
•Structural analysis of oligosaccharides
•Determining sialic acid linkage
•Glycoprotein deglycosylation
•Removing heterogeneity from glycoproteins |
| Stability : |
Stable at least 24 months when stored properly. Several days exposure to ambient temperatures will not reduce activity. |
| Storage : |
Store enzyme at 4 centigrade. |
| Storage Buffer : |
The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5). |
