Active Recombinant COVID-19 Spike protein, mFc-tagged

Cat.No. : Spike-193V
Product Overview : Recombinant COVID-19 Spike protein(YP_009724390.1)(Val16-Arg685), fused with mFc tag, was expressed in HEK293.
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  • Gene Information
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Source : HEK293
Species : COVID-19
Tag : mFc
Form : Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1, mFc Tag)(40591-V05H1), the EC50 of 2019-nCoV Spike Protein (S1, mFc Tag) is 90-240 ng/mL.
Molecular Mass : The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, mFc Tag) consists of 904 amino acids and predicts a molecular mass of 101.4 kDa.
Proteinlength : Val16-Arg685
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 90 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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