Species : |
Sars-CoV-2 |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Arg 319 - Lys 537 |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized SARS-CoV-2 S protein RBD (F490L), His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.1-2 ng/mL.Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 were added into SARS-CoV-2 S protein RBD (F490L), His Tag and SARS-CoV-2 (COVID-19) S protein RBD, His Tag: Biotinylated Human ACE2 / ACEH Protein, His,Avitag (MALS verified) binding reactions. The half maximal inhibitory concentrations (IC50) of SARS-CoV-2 S protein RBD (F490L), His Tag and SARS-CoV-2 (COVID-19) S protein RBD, His Tag are 0.20444 μg/mL and 0.78328 μg/mL respectively (Routinely tested). |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |