Active Recombinant COVID-19 Spike protein RBD (F490L), His-tagged

• Cat.No.: Spike-4727V
• Product Overview: Recombinant COVID-19 protein RBD (F490L)(Arg 319 - Lys 537), fused with His tag, was expressed in HEK293.

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Tag: His
• Protein Length: Arg 319 - Lys 537
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS-CoV-2 S protein RBD (F490L), His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.1-2 ng/mL.Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 were added into SARS-CoV-2 S protein RBD (F490L), His Tag and SARS-CoV-2 (COVID-19) S protein RBD, His Tag: Biotinylated Human ACE2 / ACEH Protein, His,Avitag (MALS verified) binding reactions. The half maximal inhibitory concentrations (IC50) of SARS-CoV-2 S protein RBD (F490L), His Tag and SARS-CoV-2 (COVID-19) S protein RBD, His Tag are 0.20444 μg/mL and 0.78328 μg/mL respectively (Routinely tested).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2