Active Recombinant COVID-19 Spike protein RBD (F490L), His-tagged
Cat.No. : | Spike-4727V |
Product Overview : | Recombinant COVID-19 protein RBD (F490L)(Arg 319 - Lys 537), fused with His tag, was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | COVID-19 |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized SARS-CoV-2 S protein RBD (F490L), His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.1-2 ng/mL.Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 were added into SARS-CoV-2 S protein RBD (F490L), His Tag and SARS-CoV-2 (COVID-19) S protein RBD, His Tag: Biotinylated Human ACE2 / ACEH Protein, His,Avitag (MALS verified) binding reactions. The half maximal inhibitory concentrations (IC50) of SARS-CoV-2 S protein RBD (F490L), His Tag and SARS-CoV-2 (COVID-19) S protein RBD, His Tag are 0.20444 μg/mL and 0.78328 μg/mL respectively (Routinely tested). |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Arg 319 - Lys 537 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] |
Official Symbol : | Spike |
Synonyms : | Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron |
Gene ID : | 43740568 |
Protein Refseq : | YP_009724390.1 |
UniProt ID : | P0DTC2 |
Products Types
◆ Lysates | ||
Spike-1741HCL | Recombinant Human coronavirus Spike cell lysate | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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