Active Recombinant COVID-19 Spike S1 protein(BA.3/Omicron), His-tagged Spike-732V

Active Recombinant COVID-19 Spike S1 protein(BA.3/Omicron), His-tagged

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Active Recombinant COVID-19 Spike S1 protein(BA.3/Omicron), His-tagged

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Specification Gene Information Related Products
Cat.No. : Spike-732V Optional Service: Optional requirements on this protein
Product Overview : Recombinant COVID-19 Spike S1 protein(BA.3/Omicron)(Val 16 - Arg 685)(QHD43416.1 (A67V,H69del,V70del,T95I,G142D,V143del,Y144del,Y145del,N211del,L212I,G339D,S371F,S373P,S375F,D405N,K417N,N440K,G446S,S477N,T478K,E484A,Q493R,Q498R,N501Y,Y505H,D614G,H655Y,N679K,P681H)), fused with His tag at the C-terminus, was expressed in HEK293.
Source : HEK293
Species : COVID-19
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized SARS-CoV-2 Spike S1, His Tag (BA.3/Omicron) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 1-63 ng/mL.
Molecular Mass : The protein has a calculated MW of 76.5 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Val 16 - Arg 685
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE :

Bioactivity-ELISA :

Gene Name : S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol : Spike
Synonyms : Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
Gene ID : 43740568
Protein Refseq : YP_009724390.1
UniProt ID : P0DTC2

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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