Active Recombinant Cynomolgus ALCAM protein(Met1-Ala526), His-tagged

• Cat.No.: ALCAM-3387C
• Product Overview: Recombinant Cynomolgus ALCAM (G7NZQ8) (Met1-Ala526) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ala526
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Cynomolgus ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc, The EC50 of mouse CD6-Fc is 0.07-0.15 μg/ml.
• Molecular Mass: The recombinant cynomolgus ALCAM comprises 510 amino acids and has a calculated molecular mass of 57.3 KDa. The apparent molecular mass of the protein is approximately 65-68 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.