Active Recombinant Cynomolgus EGFR protein(Met1-Ser645), His-tagged
Cat.No. : | EGFR-3383C |
Product Overview : | Recombinant Cynomolgus EGFR (XP_005549616.1) (Met1-Ser645) was expressed in HEK293 with a polyhistidine tag at the C-terminus. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Cynomolgus |
Tag : | C-His |
Protein length : | Met1-Ser645 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Immobilized EGF Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Cynomolgus, Recombinant (ECD, His Tag), the EC50 is 150-500 ng/mL. 2. Immobilized Anti-EGFR(MK)-IgG1 Antibody (Cetuximab) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Cynomolgus, Recombinant (ECD, His Tag), the EC50 is 2-8 ng/mL. |
Molecular Mass : | The recombinant cynomolgus EGFR consists of 632 amino acids and predicts a molecular mass of 70.1 kDa. As a result of glycosylation, it migrates as an approximately 93.8 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Products Types
◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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