Active Recombinant Cynomolgus FCGR3 Protein, His-Avi-tagged, Biotinylated

Cat.No. : FCGR3-284C
Product Overview : Biotinylated Cynomolgus CD16, His Tag is expressed from HEK293 cells. It contains AA Gly 17 - Gln 208 (Accession # Q8SPW2-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
Availability November 05, 2024
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Source : HEK293
Species : Cynomolgus
Tag : His&Avi
Predicted N Terminal : Gly 17
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Rituximab at 5 ug/mL (100 uL/well) can bind Biotinylated Cynomolgus CD16, His Tag with a linear range of 0.039-1.25 ug/mL.
Molecular Mass : The protein has a calculated MW of 25.7 kDa. The protein migrates as 36-45 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Protein length : Gly17-Gln208
Official Symbol : FCGR3
Synonyms : FCGR3
Official Symbol : FCGR3
Synonyms : FCGR3

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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