Source : |
HEK293 |
Species : |
Cynomolgus |
Tag : |
His-Avi |
Form : |
Lyophilized from 0.22 μm filtered solution in >90% as determined by SDS-PAGE.. Normally Trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Biotinylated Cynomolgus B7-H7, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human CD28H, Fc Tag with a linear range of 0.1-8 ng/mL. |
Molecular Mass : |
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 39.1 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : |
Ile 21-Asn 345 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |