Active Recombinant Cynomolgus HHLA2 protein, His-Avi-tagged, Biotinylated

• Cat.No.: HHLA2-2896C
• Product Overview: Biotinylated Recombinant Cynomolgus HHLA2 protein(Ile 21-Asn 345), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&His
• Protein Length: Ile 21-Asn 345
• Form: Lyophilized from 0.22 μm filtered solution in >90% as determined by SDS-PAGE.. Normally Trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Cynomolgus B7-H7, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human CD28H, Fc Tag with a linear range of 0.1-8 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 39.1 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin