Active Recombinant Cynomolgus IL2RB&IL2RG protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: IL2RB&IL2RG-0251C
• Product Overview: Biotinylated Recombinant Cynomolgus IL2RB&IL2RG protein(Ala27-Thr240(IL-2RB)&Leu23-Pro255(IL-2RG)), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 27-240;23-255 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag with a linear range of 0.02-0.625 μg/mL.
• Molecular Mass: Biotinylated Cynomolgus IL-2RB&IL-2RG, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB andIL-2RG, has a calculated MW of 53.0 kDa (IL-2RB) and 55.4 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag). The reducing (R) protein migrates as 70-85 kDa and 90-110 kDa respectively due to glycosylation.
• Proteinlength: Ala27-Thr240(IL-2RB)&Leu23-Pro255(IL-2RG)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Official Symbol: IL2RB