Active Recombinant Cynomolgus NKG2A & CD94 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: NKG2A & CD94-0557C
• Product Overview: Biotinylated Recombinant Cynomolgus NKG2A & CD94 protein(Ala113-Leu233(NKG2A)&Asp57-Ile179(CD94)), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 113-233;57-179 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-NKG2A Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus NKG2A&CD94, Fc,Avitag with a linear range of 0.1-16 ng/mL. Immobilized Biotinylated Cynomolgus NKG2A&CD94, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind various dilution ratio of HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE.
• Molecular Mass: Molecular CharacterizationThis protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 56.8 kDa. The protein migrates as 65-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala113-Leu233(NKG2A)&Asp57-Ile179(CD94)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin