Active Recombinant Cynomolgus/Rhesus CD274 protein(Met1-Thr239), Fc-tagged

• Cat.No.: CD274-131C
• Product Overview: Recombinant Cynomolgus / rhesus CD274 (XP_015292694.1) (Met1-Thr239) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus. Cynomolgus and Rhesus CD274 sequences are identical.

Specification

• Species: Cynomolgus/Rhesus
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Thr239
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human PD1-His at 10 μg/ml (100 μl/well) can bind Cynomolgus CD274-Fc, The EC50 of Cynomolgus CD274-Fc is 0.5-1.3 μg/ml.
• Molecular Mass: The recombinant Cynomolgus / rhesus CD274 comprises 462 amino acids and has a calculated molecular mass of 52.3 KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.